Tang, Yi

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Contact Information

Boelter Hall 5531H
(310) 825-0375
Boelter Hall 5667

Short Biography


Yi Tang received his undergraduate degree in Chemical Engineering and Material Science from Penn State University. He received his Ph.D. in Chemical Engineering from California Institute of Technology under the guidance of Prof. David A. Tirrell. After NIH postdoctoral training in Chemical Biology from Prof. Chaitan Khosla at Stanford University, he started his independent career at the University of California Los Angeles in 2004. He is currently the Chancellor Professor at the Department of Chemical and Biomolecular Engineering at UCLA, and holds joint appointments in the Department of Chemistry and Biochemistry; and Department of Bioengineering. His recent awards include the American Institute of Chemical Engineers (AIChE) Allan P. Colburn Award (2009), the American Chemical Society (ACS) Biochemical Technology Division (BIOT) Young Investigator Award (2011), the ACS Arthur C. Cope Scholar Award (2012), the EPA Presidential Green Chemistry Challenge Award (2012), NIH DP1 Director Pioneer Award (2012), the ACS Eli Lilly Award in Biological Chemistry (2014) and the Society of Industrial Microbiology and Biotechnology Charles Thom Award (2019). 

Research Interests

My research lab is interested in natural product biosynthesis and biocatalysis. In the natural product area, we are interested in elucidating biosynthetic pathways of polyketides, nonribosomal peptides and related compounds. Our goal is to understand the biochemical and structural basis of different enzymes encoded in these pathways. In particular, we are studying the biosynthesis of aromatic polyketides from Streptomyces and iteratively biosynthesized compounds from filamentous fungi. By accumulating biosynthetic knowledge and enzymatic tools, we aim towards the engineered biosynthesis of unnatural natural products through combinatorial biosynthesis. In the biocatalysis area, we build upon our knowledge from the fundamental studies and aim to discover and engineer enzymes that can be used in the synthesis and semisynthesis of pharmaceutical compounds. We have demonstrated the potential of this approach in establishing a biocatalytic approach for making the blockbuster drug simvastatin. My lab has also recently become interested in research at the interface of nanotechnology, biomaterials and drug delivery. In particular, we are conducting research towards the efficient delivery of various biological molecules to cells and model animals for applications in cancer therapy, imaging, vaccination and reprogramming. These nascent efforts are performed in collaboration with various labs.

Honors & Awards

  • Mr. & Mrs. Sun Chan Memorial Award in Organic Chemistry, the 14th International Symposium for Chinese
  • Organic Chemists (ISCOC) (2016)
  • High‐end Foreign Experts supported by State Administration of Foreign Experts Affairs P. R. China (2016)
  • California Institute of Technology Chemical Engineering Robert W. Vaughan Lectureship (2015)
  • American Chemical Society Eli Lilly Award in Biological Chemistry (2014)
  • National Institute of Health Director’s Office Pioneer Award (DP1) (2012)
  • Presidential Green Chemistry Challenge Award, the United States Environmental Protection Agency (2012)
  • American Chemical Society (ACS) Arthur C. Cope Scholar Award (2012)
  • The Saville Lecturer at Department of Chemical and Biological Engineering, Princeton University (2011)
  • American Chemical Society Division of Biochemical Technology (BIOT) Young Investigator Award (2011)
  • National Academy of Engineering (NAE) Frontiers in Engineering Invitee (2010)
  • Society for Industrial Microbiology (SIM) Young Investigator Award (2010)
  • American Institute of Chemical Engineers (AIChE) Allan P. Colburn Award (2009)
  • Sloan Research Fellowship (2009)
  • Department of Defense Breast Cancer Research Program (BCRP) Concept Award (2008)
  • The Camille Dreyfus Teacher Scholar Award (2008)
  • The David and Lucile Packard Foundation Fellowship (2007)
  • UCLA Faculty Career Development Award (2007)
  • Presidential Early Career Award in Science and Engineering (PECASE) (2006)
  • National Science Foundation CAREER Award (2006)
  • UCLA AIChE Student Chapter Professor of the Year Award (2005, 2006)
  • American Heart Association National Scientist Development Grant Award (2005‐2009)
  • National Research Service Award, National Institute of Health (2002‐2004)
  • Whitaker Graduate Research Fellowship in Biomedical Engineering (1997‐2002)
  • Constantin Economou Award for Excellency in Graduate Research (1999)
  • Elizabeth and Holmes Memorial Scholarship (1993‐1997)
  • Dow Chemical Co. Outstanding Student in Material Science and Engineering (1995)
  • The 1934 Penn State Alumni Scholarship Award (1995)




199. YY, in preparation.

198. ZZ, in preparation.

197. MO, in preparation.

196. MC, 2020, submitted.

195. UB, 2020, submitted.

194. Hai, Y., Huang, A., Tang, Y.* "Biosynthesis of Amino Acid-Derived alpha-Pyrones by an NRPS-NRPKS Hybrid Megasynthetase in Fungi." J. Nat. Prod. 2020accepted. (invited article for a special issue dedicated to Prof. Jon Clardy) Link

193. Yee, D. A.; Kakule, T.; Cheng, W.; Chen, M.; Chong, C.; Hai, Y.; Hang, L.; Hung, Y.; Liu, N.; Ohashi, M.; Okorafor, I.; Song, Y.; Tang, M-C.; Zhang, Z.; Tang, Y.* "Genome Mining of Alkaloidal Terpenoids from a Hybrid Terpene and Nonribosomal Peptide Biosynthetic Pathway." J. Am. Chem. Soc. 2020, 142, 710-714. PMCID: PMC7000236 Link

192. Yan, Y., Liu, N., Tang, Y.* "Recent developments in self-resistance gene directed natural product discovery." Nat. Prod. Rep, 2020, accepted. NIHMSID:NIHMS1069080. Link

191. Hung, Y-S., Zhao, Z-Y., Soohoo, A.Tang, Y.* "Genome Mining of Fungi" Comprehensive Natural Products III: Chemistry and Biology2020, Volume 6, accepted .

190. Go, E., Tang, Y.* "Fungal Highly Reducing Polyketide Synthases and Associated Natural Products" Comprehensive Natural Products III: Chemistry and Biology2020, Volume 1Link



189. Liu, L., Tang, M-C., Tang, Y.* “Fungal Highly Reducing Polyketide Synthases Biosynthesize Salicylaldehydes that are Precursors to Epoxycyclohexenol Natural Products.” J. Am. Chem. Soc2019, 141, 19538-19541. PMCID: PMC6924165 Link

188. Hai, Y., Jenner, M., Tang, Y.* “Complete Stereoinversion of L-tryptophan by a Fungal Single Module Nonribosomal Peptide Synthetase.” J. Am. Chem. Soc2019, 141, 8198-8206. PMCID: PMC6927398 Link

187. Kishimoto, S., Tsunematsu, Y., Matsushita, T., Hara, K., Hashimoto, H., Tang, Y., Watanabe, K.* "Functional and Structural Analyses of trans C-Methyltransferase in Fungal Polyketide Biosynthesis." Biochemistry, 2019, 58, 3933-3937. Link

186. Cai, Y., Hai, Y., Ohashi, M., Jamieson, C. S., Garcia-Borras, M., Houk, K. N.*, Zhou, J.*, Tang, Y.* "Structural Basis for Stereoselective Dehydration and Hydrogen-Bonding Catalysis by the SAM-Dependent Pericyclase LepI." Nature Chemistry, 2019, 11, 812–820. PMCID: PMC6708486 Link BioRxivDOI: https://doi.org/10.1101/491761. Link

185. Yee, D. A., DeNicola, A. B., Billingsley, J. M., Creso, J. G., Subrahmanyam, V.Tang, Y.* " Engineered Mitochondrial Production of Monoterpenes in Saccharomyces cerevisiae." Metab. Eng. 2019, 55, 76-84. PMCID: PMC6717016 Link

184. Billingsley, J. M., Anguiano, J. L.Tang, Y.* "Production of semi-biosynthetic nepetalactone in yeast." J. Ind. Microb. Biotechnol2019, 46, 1365-1370. PMCID: PMC6927397 Link

183. Tang, M-C., Fischer, C. R., Chair, J. V., Tan, D., Suresh, S., Chu, A., Miranda, M., Smith, J., Zhang, Z., Garg, N. K., St. Onge, R. P.*, Tang, Y.* “Thioesterase-catalyzed aminoacylation and thiolation of polyketides in fungi.” J. Am. Chem. Soc2019, 141, 8198-8206. PMCID: PMC6589186 Link

182. Hai, Y., Huang, A.Tang, Y.* "Structure-guided function discovery of an NRPS-like glycine betaine reductase for choline biosynthesis in fungi." Proc. Natl. Acad. Sci. U.S. A. 2019, 116, 10348-10353. PMCID: PMC6535007 Link BioRxiv2019, DOI: https://doi.org/10.1101/559534 Link

181. Zhang, Z., Jamieson, C. S., Zhao, Y-L., Li, D., Ohashi, M.*, Houk, K. N.*, Tang, Y.*, “Enzyme-catalyzed inverse-electron demand Diels-Alder reaction in the biosynthesis of antifungal ilicicolin H.” J. Am. Chem. Soc2019, 141, 5659–5663PMCID: PMC6585442 Link

180. Zhao, Z-Y., Yin, Y., Hung, Y., Tang, Y.* "Genome Mining Reveals Neurospora crassa can Produce the Salicylaldehyde Sordarial." J. Nat. Prod.2019, 8, 1029-1033. PMCID: PMC6933945 Link

179. Chen, M., Liu, Q., Gao, S-S., Young, A. E., Jacobsen, S. E., Tang, Y.*, "Genome mining and biosynthesis of a polyketide from a biofertilizer fungus that can facilitate reductive iron assimilation in plant."Proc. Natl. Acad. Sci. U. S. A. 2019, 116, 5499-5504 PMCID: PMC6431147 Link

178. Tan, D., Jamieson, C. S., Ohashi, M., Tang, M-C.*, Houk, K. N.*, Tang, Y.*, “Genome-mined Diels-Alderase catalyzes formation of the cis-octahydrodecalins of varicidin A and B.” J. Am. Chem. Soc2019, 141, 769-773PMCID: PMC6475471 Link

177. DeNicola, A. B., Tang, Y. "Therapeutic approaches to treat human spliceosomal diseases." Curr. Opin. Biotechnol. 2019, 60, 72-81Link

176. Bond, C. M., Tang, Y.* "Engineering Saccharomyces cerevisiae for production of simvastatin " Metabolic Engineering, . 2019, 51, 1-8PMCID: PMC6348118 Link

175. Jamieson, C. S., Ohashi, M., Liu, F., Tang, Y.*, Houk, K. N.* “The Expanding World of Biosynthetic Pericyclases: Cooperation of Experiment and Theory for Discovery.” Nat. Prod. Rep. 2019, 36, 698-713. PMCID: PMC6461539 Link



174. Yan, Y., Liu, Q., Jacobsen, S. E.*, Tang, Y.*, " The Impact and Prospect of Natural Product Discovery in Agriculture." EMBO Reports, 2018DOI 10.15252/embr.201846824. (Invited opinion on Science and Society). PMCID: PMC6216283 Link

173. Kishimoto, S., Hara, K. Hashimoto, H., Hirayama, Y., Champagne, P. A., Houk, K. N., Tang, Y., Watanabe, K.* "Enzymatic one-step ring contraction for quinolone biosynthesis." Nature Communications, 2018, Article number: 2826 . Link

172. Yan, Y,# Liu, Q.#, Zang, X.#, Yuan, S., Bat-Erdene, U., Nguyen, C. Gan, J., Zhou, J.*, Jacobsen, S. E.*Tang, Y.*, " Resistance-Gene Directed Discovery of a Natural Product Herbicide with a New Mode of Action." Nature, 2018, 559, 415-418. PMCID: PMC6097235 Link

171. Ippoliti, F. M. Barber, J. S., Tang, Y. Garg, N. K*, “Synthesis of 8-Hydroxygeraniol.” J. Org. Chem., 2018, 83, 11323–11326Link

170. Tang, M-C.#, Zou, Y.#, Yee, D. A., Tang, Y.*, "Identification of the Pyranonigrin A Biosynthetic Gene Cluster by Genome Mining in Penicillium thymicola IBT5891." AICHE J., 2018, 64, 4182-4186. (Invited article for special issue Honoring Jay Bailey) PMCID: PMC6777573 Link

169. Gao, S-S., Zhang, T., Garcia-Borras, M., Hung, Y-S., Billingsley, J. M., Houk, K. N.*, Hu, Y.*Tang, Y.“Biosynthesis of Heptacyclic Duclauxins Requires Extensive Redox Modifications of the Phenalenone Aromatic Polyketide” J. Am. Chem. Soc. 2018, 140, 6991–6997MCID: PMC6309916 Link

168. Li, L.#, Tang, M-C.#, Tang, S., Gao, S., Soliman, S., Hang, L., Xu, W., Ye, T., Watanabe, K., Tang, Y.*, "Genome Mining and Assembly-Line Biosynthesis of the UCS1025A Pyrrolizidinone Family of Fungal Alkaloids." J. Am. Chem. Soc2018, 140, 2067-2071. PMCID: PMC5817883 Link

167. Harvey, C.#, Tang, M-C.#, (Stanford/UCLA U01 Team), Tang, Y., Hillenmeyer, M. E.* “HEx: a heterologous expression platform for the discovery of fungal natural products.” bioRxiv2018, https://doi.org/10.1101/247940. Link Science Advances, 2018, DOI: 10.1126/sciadv.aar5459. Link

166. Hai, Y., Tang, Y.*, “Biosynthesis of long-chain N-acyl amide by a truncated PKS-NRPS hybrid megasynthase in fungi.” J. Am. Chem. Soc2018, 1401271–1274. PMCID: PMC5817892 Link

165. Bai, J., Mu, R., Dou, M., Yan, D., Liu, B., Wei, Q., Wan, J., Tang, Y., Hu, Y.* “Epigenetic modification in histone deacetylase deletion strain of Calcarisporium arbuscula leads to diverse diterpenoids.” Acta Pharmaceutica Sinica B, 2018, 8(4):687-697 . Link

164. Walsh, C. T.*, Tang, Y.* "Recent Advances in Enzymatic Complexity Generation: Cyclization Reactions." Biochemistry 2018, 57, 3087–3104. PMCID: PMC5988943 Link

163. Walsh, C. T.*, Tu, B. P.*, Tang, Y.*, "Eight Kinetically Stable but Thermodynamically Activated Molecules that Power Cell Metabolism." Chem. Rev. 2018, 118, 1460-1494. PMCID: PMC5831524 Link



162. Billingsley, J. M., DeNicola, A. B., Barber, J. S., Tang, M-C., Horecka, J., Chu, A., Garg, N. K., Tang, Y.*, “Engineering the Biocatalytic Selectivity of Iridoid Production in Saccharomyces cerevisiae.” Metab. Eng2017, 44, 117-125. PMCID: PMC5705256. Link

161. Tang, M-C., Cui, X., He, X., Ding, Z., Zhu, T., Tang, Y.*, Li, D.* “Late-Stage Terpene Cyclization by an Integral Membrane Cyclase in the Biosynthesis of Isoprenoid Epoxycyclohexenone Natural Products.” Org. Lett. 2017, 19, 5376–5379. PMCID: PMC5682560 Link

160. Ohashi, M., Liu, F., Hai, Y., Chen, M., Tang, M-C., Yang, Z., Sato, M., Watanabe, K.*, Houk, K. N.*Tang, Y.*, “SAM-Dependent Enzyme-Catalysed Pericyclic Reactions in Natural Product Biosynthesis.” Nature2017, 549, 502–506. PMCID: PMC5679075 Link.

159. Zhang, Y., Zou, Y., Brock, N. L., Huang, T., Lan, Y., Wang, X., Deng, Z., Tang, Y., Lin, S.* "Characterization of 2-Oxindole Forming Heme Enzyme MarE, Expanding the Functional Diversity of the Tryptophan Dioxygenase Superfamily." J. Am. Chem. Soc. 2017,139, 11887–11894 Link

158. Hang, L., Tang, M-C., Harvey, C. J. B., Page, C. G., Li, J., Hung, Y., Liu, N., Hillenmeyer, M. E., Tang, Y.*, "Reversible Product Release and Recapture by a Fungal Polyketide Synthase Using a Carnitine Acyltransferase Domain." Angew. Chem. Intl. Ed2017, 56, 9556-9560. PMCID: PMC5580239 Link

157. Liu, N., Hung, Y., Gao, S-S., Hang, L., Zou, Y., Chooi, Y-H.*, Tang, Y.*, "Identification and Heterologous Production of a Benzoyl-Primed Tricarboxylic Acid Polyketide Intermediate from the Zaragozic Acid A Biosynthetic Pathway." Org. Lett. 2017, 19, 3560–3563. PMCID: PMC5673471 Link

156. Sato, M., Dander, J. E., Sato, C., Hung, Y., Gao, S-S, Tang, M-C, Hang, L., Winter, J. M., Garg, N. K.*, Watanabe, K.*, Tang, Y.*, “Collaborative Biosynthesis of Maleimide- and Succinimide-Containing Natural Products by Fungal Polyketide Megasynthases.” J. Am. Chem. Soc2017, 139, 5317–5320. PMCID: PMC5673468 Link

155. Bai, J., Yan, D., Zhang, T., Guo, Y,, Liu, Y., Zou, Y., Tang, M-C., Liu, B., Wu, Q., Yu, S.* Tang, Y.*, Hu, Y.* "A Cascade of Redox Reactions Generates Complexity in the Biosynthesis of the Protein Phosphatase-2 Inhibitor Rubratoxin A", Angew. Chem. Intl. Ed. 2017, 56, 4782–4786Link

154. Gao, S-S., Garcia-Borras,M., Barber, J. S., Hai, Y., Duan, A., Garg, N. K.*, Houk, K. N.*Tang, Y.*, “Enzyme-Catalyzed Intramolecular Enantioselective Hydroalkoxylation.” J. Am. Chem. Soc. 2017, 139, 3639-3642PMCID: PMC5474393 Link

153. Zou, Y., Garcia-Borras, M., Tang, M-C., Hirayama, Y., Li, D., Li, L., Watanabe, K., Houk, K. N.*Tang, Y.*, “Enzyme-Catalyzed Cationic Epoxide Rearrangements in Quinolone Alkaloid Biosynthesis.” Nat. Chem. Biol2017, 13, 325-332. PMCID: PMC5310975 Link

152. Tang, M-C.,# Zou, Y.,# Watanabe, K., Walsh, C. T.*, Tang, Y.* "Oxidative Cyclization in Natural Product Biosynthesis." 2017Chem. Rev. 117, 5226–5333PMCID: PMC5406274 Link



151. Li, L. Yu, P., Tang, M-C., Zou, Y., Gao, S-S., Hung, Y-S., Zhao, M., Watanabe, K.*, Houk, K. N.*, Tang, Y.* "Biochemical Characterization of a Eukaryotic Decalin-Forming Diels-Alderase." J. Am. Chem. Soc. 2016, 138, 15837–15840 PMCID: PMC5323083 Link

150. Zhang, J., Liu, N.#, Cacho, R. A.#, Gong, Z., Liu, Z., Qin, W., Tang, C., Tang, Y.*, Zhou, J.*, “Structural Basis of Nonribosomal Peptide Macrocyclization in Fungi .” Nat. Chem. Biol2016, 12, 1001-1003. PMCID: PMC5110376 Link

149. Yu, X., Liu, F., Zou, Y., Tang, M-C. Hang, L., Houk, K. N.*, Tang, Y.* "Biosynthesis of Strained Piperazine Alkaloids – Uncovering the Concise Pathway of Herquline A."J. Am. Chem. Soc. 2016, 138, 13529–13532PMCID: PMC5378693 Link

148. Hang, L.#, and Liu, N.#, Tang, Y.* "Coordinated and Iterative Enzyme Catalysis in Fungal Polyketide Biosynthesis." ACS Catalysis 2016, 6, 5935–5945 (invited perspective). PMCID: PMC5436725 Link

147. Grandner, J. M., Cacho, C. A., ., Tang, Y. * Houk, K. N.* “Mechanism of the P450-Catalyzed Oxidative Cyclization in the Biosynthesis of Griseofulvin.” ACS Catal. 2016, 6, 4506–4511. Link

146. Yamamoto, T., Tsunematsu, Y., Hara, K., Suzuki, T., Kishimoto, S., Kawagishi, H., Noguchi, H., Hashimoto, H., Tang, Y., Hotta, K., Watanabe, K.* "Oxidative trans to cis Isomerization of Olefins in Polyketide Biosynthesis." Angew. Chem. Intl. Ed. 2016, 55, 6207-6210. PMCID: PMC5089960 Link

145. Gao, S-S., Duan, A., Xu, W., Yu, P., Hang, L., Houk, K. N.*, Tang, Y.* "Phenalenone Polyketide Cyclization Catalyzed by Fungal Polyketide Synthase and Flavin-Dependent Monooxygenase."J. Am. Chem. Soc. 2016, 138, 4249–4259Link

144. Lin, H-C., McMahon, T., Patel, A., Corsello, M., Simon, A., Xu, W., Zhao, M., Houk, K. N.*, Garg, N. K.*, Tang, Y.*. "P450-Mediated Coupling of Indole Fragments to Forge Communesin and Unnatural Isomers."J. Am. Chem. Soc. 2016, 138, 4002–4005. Link

143. Sato, M., Winter, J. M., Kishimoto, S., Noguchi, H., Tang, Y., Watanabe, K.* " Combinatorial Generation of Chemical Diversity by Redox Rnzymes in Chaetoviridin Biosynthesis." Org. Lett. 2016, 18, 1446-1449Link

142. Billingsley, J. M.#, DeNicola, A. B.#, Tang, Y.* “Technology development for natural product biosynthesis in Saccharomyces cerevisiae.” Curr. Opin. Biotechnol. 2016, 42, 74-83(Pharmaceutical Biotechnology issue) PMCID: PMC5026534 Link

141. Oikawa, H.*, Tang, Y.* "Exploring the Diversification Mechanism of Sskeletal Construction in Representative Natural Product Families." J. Antibiot. 2016, 69, 471-472. (Editorial on Special Issue honoring Prof. David Cane) Link

140. Zhao M., Lin, H.-C. Tang, Y.* “Biosynthesis of the α-Nitro-containing Cyclic Tripeptide Psychrophilin.” J. Antibiot2016, 69, 571-573. (Invited publication for Special Issue honoring Prof. David CaneLink

139. Bond, C. M.#, Tang, Y.*, Li. L.#, “Saccharomyces cerevisiae as a Tool for Mining, Studying and Engineering Fungal Polyketide Synthases.” Fungal Genet. Biol. 2016, 89, 52-61Link

138. Cacho, R. C., Tang, Y.* “Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro.” Methods Mol. Biol. 2016, 1401,103-119. Link

137. Cochrane, R., Sanichar, R., Lambkin, G., Xu, W., Tang, Y., Vederas, J. C.*, “Identification and Reconstitution of the Polyketide Synthases Responsible for Biosynthesis of the Anti-Malarial Agent, Cladosporin.” Angew. Chem. Intl. Ed2016, 55, 664-668. Link

136. Xu, W., Raetz, L. B., Wang, P., Tang, Y.* “An ATP-Dependent Ligase Catalyzes the Fourth Ring Cyclization in Tetracycline Biosynthesis.” Tetrahedron 2016, 72, 3599-3604(Invited submission for Special Issue on Natural Product-Inspired Approaches to Combat Bacteria) Link

135. Noey, E. L., Tibrewal, N., Jimenez-Oses, G., Osuna, S., Park, J., Bond, C., Casio, D., Liang, J., Zhang, X., Huisman, G., Tang, Y.*, Houk, K. N.*, “Origins of Stereoselectivity in Evolved Ketoreductases.” Proc. Natl. Acad. Sci., U. S. A., 2016, 112, E7065–E7072, doi: 10.1073/pnas.1507910112LinkNews



134. Cacho, R. A., Thuss, J., Xu, W., Sanichar, R., Gao, Z., Nguyen, A., Vederas, J. C.*, Tang, Y.* “Understanding Programming of Fungal Iterative Polyketide Synthases: the Biochemical Basis for Regioselectivity by the Methyl-transferase Domain in the Lovastatin Megasynthase.” J. Am. Chem. Soc. 2015, 137, 15688-15691. Link

133. Guo, Y-Y., Li, H., Zhou, Z-X., Mao, X-M., Tang, Y., Chen, X., Jiang, X-H., Liu, Y, Jiang, H., Li, Y-Q.*, “ Identification and Biosynthetic Characterization of Natural Aromatic Azoxy Products from Streptomyces chattanoogensis L10.” Org. Lett. 2015, 17, 6114-6117Link

132. Tang, M-C., Lin, H-C., Li, D., Zou, Y., Li, J., Xu, W., Cacho, R. A., Hillenmeyer, M., Garg, N. K., Tang, Y.* “Discovery of Unclustered Fungal Indole Diterpene Biosynthetic Pathways through Combinatorial Pathway Reassembly in Engineered Yeast.” J. Am. Chem. Soc. 2015, 137, 13724–13727. Link

131. Cochrane, R., Gao, Z., Lambkin, G., R., Xu, W., Winter, J. M., Marcus, S. L., Tang, Y., Vederas, J. C.*, “Comparison of 10,11-dehydrocurvularin polyketide synthases from Alternaria cinerariae and Aspergillus terreus highlights key structural motifs.” ChemBioChem 2015, 16, 2479-2483. Link

130. Sato, M., Yagishita, F., Mino, T., Uchiyama, N., Patel, A., Chooi, Y., Goda, Y., Xu, W., Noguchi, H., Yamamoto, T., Hotta, K., Houk, K. N., Tang, Y., Watanabe, K.* “Involvement of Lipocalin-like CghA in Decalin-Forming Stereoselective Intramolecular [4+2] Cycloaddition.” ChemBioChem 2015, 16, 2294-2298Link

129. Mao, X-M., Zhan, Z-J., Grayson, M. N., Tang, M-C., Xu, W., Li, Y-Q., Yin, W-B., Lin, H-C., Chooi, Y., Houk, K. N., Tang, Y.*, “Efficient Biosynthesis of Fungal Polyketides Containing the Dioxabicyclo-octane Ring System.” J. Am. Chem. Soc. 2015, 137, 11904-11907. Link

128. Medema, M. H. et al. “Minimum Information about a Biosynthetic Gene Cluster.” Nat. Chem. Biol. 2015, 11, 625-631. Link

127. Winter, J. M., Cascio, D.; Dietrich, D.; Sato, M.; Watanabe, K.; Sawaya, M.; Vederas, J. C.; Tang, Y*, “Biochemical and Structural Basis for Controlling Chemical Modularity in Fungal Polyketide Biosynthesis.”. J. Am. Chem. Soc. 2015, 137, 9885-9893. Link

126. Mao, X-M., Xu, W., Li, D., Yin, W-B., Chooi, Y-H., Li, Y-Q., Tang, Y*, Hu, Y.* “Epigenetic Genome Mining of an Endophytic Fungus Leads to Pleiotropic Biosynthesis of Natural Products.” Angew. Chem. Intl. Ed. 2015, 54, 7592–7596Link.

125. Zou, Y., Zhan, Z., Li, D., Tang, M-C., Cacho, R. A., Watanabe, K., Tang, Y.* “Tandem Prenyltransferases Catalyze Isoprenoid Elongation and Complexity Generation in Biosynthesis of Quinolone Alkaloids.” J. Am. Chem. Soc. 2015, 137, 4980-4983. Link

124. Mao, X-M., Luo, S., Zhou, R-C., Wang, F., Yu, P., Sun, N., Chen, X-X., Tang, Y., Li, Y-Q.* “Transcriptional Regulation of the Daptomycin Gene Cluster in Streptomyces roseosporus by an Autoregulator, AtrA.” J. Biol. Chem. 2015, 290, 7992-8001. Link

123. Lin, H-C., Chiou, G., Chooi, Y., McMahon, T. C., Xu, W., Garg, N. K.*, Tang, Y.* “Elucidation of the Concise Biosynthetic Pathway of the Communesin Indole Alkaloids.” Angew. Chem. Intl. Ed. 2015, 54, 3004-3007. (VIP paper) Link

122. Cacho, R. A., Tang, Y., Chooi, Y.*, “Next-generation Sequencing Approach for Connecting Secondary Metabolites to Biosynthetic Gene Clusters in Fungi.” Front. Microbiol2015, DOI: 10.3389/fmicb.2014.00774. Link

121. Soliman, S., Tang, Y.* “Natural and Engineered Production of Taxadiene with Taxadiene Synthase.” Biotechnol. Bioeng. 2015, 112, 229-235. Link



120. Zou, Y., Xu, W., Tsunematsu, Y., Tang, M-C., Watanabe, K., Tang, Y. * “Methylation-dependent Acyl Transfer between Polyketide Synthase and Nonribosomal Peptide Synthetase Modules in Fungal Natural Product Biosynthesis.” Org. Lett. 2014, 16, 6390-6393. Link

119. Winter, J. M., Tang, Y. * “Natural products: Getting a handle on peptides.” Nat. Chem. 2014, 6, 1037–1038. (Invited Commentary) Link

118. Zhao, M.; Liu, Y.; Hsieh, R.; Wang, N.; Tai, W.; Joo, K.; Wang, P.; Gu, Z.; Tang, Y.* “Clickable Protein Nanocapsules for Targeted Delivery of Recombinant p53 Protein.” J. Am. Chem. Soc. 2014, 136, 15319–15325. Link

117. Xu, W., Gavia, D., Tang, Y.* “Biosynthesis of Fungal Indole Alkaloids” NatProd. Rep. 2014, 31, 1474-1487. Link

116. Tsunematsu, Y.; Fukutomi, M.; Saruwatari, T.; Noguchi, H.; Hotta, K.; Tang, Y. , Watanabe, K.* “Elucidation of the Pseurotin Biosynthetic Pathway Reveals Trans-Acting C-Methyltransferase and Source of Chemical Diversity Generation.” Angew. Chemie. Intl. Ed. 2014, 53, 8475-8479. Link

115. Zabala, A. O., Chooi, Y., Choi, M., Lin, H-C., Tang, Y.* “Fungal Polyketide Synthase Product Chain-Length Control by Partnering Thiohydrolase” ACS. Chem. Biol2014, 9, 1576-1586. Link

114. Hu, Y., Dietrich, D., Xu, W., Thuss, J. A., Wang, J., Yin, W-B., Qiao, K., Vederas, J. C.*, Tang, Y.* “A Carbonate-Forming Baeyer-Villiger Monooxygenase.” Nat. Chem. Biol2014, 10, 552–554. Link

113. Jiménez-Osés, G.; Osuna, S.; Gao, X.; Sawaya, M. R.;Gilson, L.; Collier, S. J.;Huisman, G. W.*; Yeates, T. O.*; Tang, Y.*; Houk, K. N.* “The Role of Distant Mutations and Allosteric Regulation on LovD Active Site Dynamics.” Nat. Chem. Biol2014, 10, 431–436. Link

112. Lin, H-C; Tsunematsu, Y.; Dhingra, S.; Xu, W.; Fukutomi, M.; Chooi, Y.; Cane, D. E.; Calvo, A.; Watanabe, K.; Tang, Y.* “Generation of Complexity in Fungal Terpene Biosynthesis: Discovery of a Multifunctional Cytochrome P450 in the Fumagillin Pathway.” J. Am. Chem. Soc2014, 136, 4426-4436Link

111. Mao, X, Ren, N., Sun, N., Wang, F., Zhou, R., Tang, Y., Li, Y.* “Proteasome involvement in a complex cascade mediating SigT degradation during differentiation of Streptomyces coelicolor.” FEBS Lett2014, 588, 608-613. Link

110. Tibrewal, N.*, Tang, Y.* “Biocatalysts for Natural Product Biosynthesis.” Annu. Rev. Chem. Bio. Eng2014, 5, 347-366. Link

109. Li, L. Wang, P., Tang, Y.* “C-Glycosylation of Anhydrotetracycline Scaffold with SsfS6 from the SF2575 Biosynthetic Pathway.” J. Antibiot. 2014, 67, 65-70(Chris Walsh Issue) Link

108. Chooi, Y., Hong, Y., Cacho, R. A., Tantillo, D. J.*, Tang, Y.* “A Cytochrome P450 Serves as an Unexpected Terpene Cyclase during Fungal Meroterpenoid Biosynthesis.” J. Am. Chem. Soc. 2013, 135, 16805–16808Link

107. Dhingra, S., Lee, A., Lin, H-S., Tang, Y., Rokas, A., Calvo. A. M.*, “The fumagillin gene cluster, an example of thousands of genes under veA control in Aspergillus fumigatus.” PLoS ONE2013, 8(10): e77147. doi:10.1371/journal.pone.0077147. Link

106. Liu, Y., Ding, G., Li, Y., Qu, J., Ma, S., Lv, H., Liu, Y., Wang, W., Dai, J., Tang, Y., Yu, S.* “Structures and Absolute Configurations of Penicillactones A–C from an Endophytic Microorganism, Penicillium dangeardii Pitt.” Org. Lett. 2013, 15, 5206–5209. Link

105. Cacho, R. A. Chooi, Y., Zhou, H., Tang, Y.* “Complexity Generation in Fungal Polyketide Biosynthesis: a Spirocycle-Forming P450 in the Concise Pathway to the Antifungal Drug Griseofulvin.” ACS Chem. Biol. 2013, 8, 2322–2330Link

104. Gao, X., Jiang, W., Jiménez-Osés, G., Choi, M. S., Houk, K. N., Tang, Y.*, Walsh, C. T.*, “An Iterative, Bimodular Nonribosomal Peptide Synthetase that Converts Anthranilate and Tryptophan into Tetracyclic Asperlicins.” Chem. Biol. 2013, 20, 870-878Link See Former Member (both Tang and Walsh!) Wenjun Zhang’s paper in the same issue.

103. Winter, J. M, Chiou, G., Bothwell, I. Xu, W., Garg, N. K., Luo, M. K., Tang, Y.* “Expanding the Structural Diversity of Polyketides by Exploring the Cofactor Tolerance of an Inline Methyltransferase Domain.” Org. Lett.. 2013, 15, 3774–3777Link

102. Yin, W. B., Chooi, Y., Cacho, R. A., Smith, A. R., White, T. C., Tang, Y.* “Discovery of Cryptic Polyketide Metabolites from Dermatophytes using Heterologous Expression in Aspergillus nidulans.” ACS Syn. Biol. 2013, 2, 629–634. Link.

101. Xu, W.Chooi, Y., Choi, J. W., Li, S., Vederas, J. C., Da Silva, N. A., Tang, Y.* “Finding the Missing Link in Lovastatin Biosynthesis: LovG is the Thioesterase Required for Dihydromonacolin L Release and Lovastatin Nonaketide Synthase Turnover.” Angew. Chem. Intl. Ed. 2013, 52, 6472-6475Link Highlighted by Nature Chem. Biol.

100. Wang, P., Bashiri, G., Gao, X., Sawaya, M. R. Tang, Y. * “Uncovering the Enzymes that Catalyze the Final Steps in Oxytetracycline Biosynthesis.” J. Am. Chem. Soc. 2013, 135, 7138-7141. Link

99. Walsh, C. T.*, Haynes, S. W., Ames, B. D., Gao, X., Tang, Y. “Short Pathways to Complexity Generation: Fungal Peptidyl Alkaloid Multicyclic Scaffolds from Anthranilate Building Blocks.” ACS Chem. Biol2013, 8, 1366–1382. Link

98. Wei, W., Du, J., Li, J., Yan, M., Zhu, Q., Jin, X., Zhu, X., Hu, Z., Tang, Y.*, Lu, Y.* “Construction of Robust Enzyme Nanocapsules for Effective Organophosphate Decontamination, Detoxification, and Protection.” Adv. Mater. 2013, 25, 2212-2218. Link

97. Lin, H-S., Chooi, Y., Dhingra, S., Xu, W., Calvo, A. M., Tang, Y.* “The Fumagillin Biosynthetic Gene Cluster in Aspergillus fumigatus Encodes a Cryptic Terpene Cyclase Involved in the Formation of b-trans-Bergamotene.” J. Am. Chem. Soc. 2013, 135, 4616-4619. Link

96. Jiang, W., Cacho, R. A., Chiou, G. Garg. N. K., Tang, Y.*, Walsh, C. T.*, “EcdGHK Are Three Tailoring Iron Oxygenases for Amino Acid Building Blocks of the Echinocandin Scaffold.” J. Am. Chem. Soc. 2013, 135, 4457-4466Link

95. Chooi, Y., Fang, J., Liu, H., Filler, S., Wang, P., Tang, Y.* “Genome Mining of a Prenylated and Immunosuppressive Polyketide from Pathogenic Fungi.” Org. Lett. 2013, 15, 780-783Link

94. Gao, Z.+, Wang, J.+, Norwqay, A. K., Qiao, K., Tang, Y.*, Vederas, J. C.* “Investigation of Fungal Iterative Polyketide Synthase Functions Using Partially Assembled Intermediates.” J. Am. Chem. Soc. 2013, 135, 1735-1738. Link

93. Cacho, R. A., Tang, Y.* “Bringing Protein Engineering and Natural Product Biosynthesis Together.” Chem, Biol. 2013, 20, 3-5. (Commentary) Link

92. Haynes, S. W., Gao, X., Tang, Y.*, Walsh, C. T.*, “Complexity Generation in Fungal Peptidyl Alkaloid Biosynthesis: a Two Enzyme Pathway to the Hexacyclic MDR Export Pump Inhibitor Ardeemin.” ACS Chem. Biol2013, 8, 741-748. Link

91. Zhao, M., Hu, B., Gu, Z., Joo, K., Wang, P.*, Tang, Y.* “Degradable Polymeric Nanocapsule for Efficient Intracellular Delivery of a High Molecular Weight Tumor-Selective Protein Complex.” Nano Today. 2013, 8, 11-20Link

90. Xu, W., Qiao, K., Tang, Y.* “Structural Analysis of Protein-Protein Interactions in Polyketide Biosynthesis.” Crit. Rev. Biochem. Mol. Biol2012, 48, 98-122. Link

89. Winter, J. M. Sato, M., Sugimoto, S., Chiou, G., Garg, N. K., Tang, Y.*, Watanabe, K.*, “Identification and Characterization of the Chaetoviridin and Chaetomugilin Gene Cluster in Chaetomium globosum Reveal Dual Functions of an Iterative Highly-Reducing Polyketide Synthase.” J. Am. Chem. Soc. 2012, 134, 17900–17903. Link

88. Haynes, S. W., Gao, X., Tang, Y.*, Walsh, C. T.*, “Assembly of Asperlicin Peptidyl Alkaloids from Anthranilate and Tryptophan: A Two-Enzyme Pathway Generates Hepta-cyclic Scaffold Complexity in Asperlicin E.” J. Am. Chem. Soc., 2012, 134, 17444–17447. Link

87. Cacho, R. A., Jiang, W., Chooi, Y., Walsh, C. T.*, Tang, Y.* “Identification and Characterization of the Echinocandin B Biosynthetic Gene Cluster from Emericella rugulosa NRRL 11440.” J. Am. Chem. Soc. 2012, 134, 16781–16790. Link

86. Chooi, Y.*, Tang, Y.* “Navigating the Fungal Polyketide Chemical Space: From Genes to Molecules.” J. Org. Chem. 2012, 77, 9933–9953. (invited perspective) Link

85. Wang, P., Kim. W., Pickens, L. B., Gao, X., Tang, Y.* “Heterologous Expression and Manipulation of Three Tetracycline Biosynthetic Pathways.” Angew. Intl. Chem. Ed. 2012, 51, 11136–11140. (selected as Very Important Paper). Link

84. Gao, X., Haynes, S. W., Ames, B. D., Wang, P., Vien, L. P., Walsh, C. T.*Tang, Y.* “Cyclization of Fungal Nonribosomal Peptides Catalyzed by a Terminal Condensation-Like Domain.” Nat. Chem. Biol. 2012, 8, 823-830. Link

83. Zabala, A. O., Xu, W., Chooi, Y.*Tang, Y.* “Discovery and Characterization of a Silent Gene Cluster that Produces Azaphilones from Aspergillus niger ATCC 1015 Reveal a Hydroxylation-Mediated Pyran-Ring Formation.” Chem. Biol. 2012, 19, 1049–1059. Link

82. Ames, B. D., N., Chi, Smith, P., Ma, S. M., Wong, E., Wong, S., Xie, X., Li, J. W., Vederas, J. C., Tang, Y.*, Tsai, S. C.* “Biosynthesis of Lovastatin: Crystal Structure and Biochemical Studies of LovC, a Trans-acting Polyketide Enoyl Reductase.” Proc. Natl. Acad. Sci. U. S. A2012, 109, 11144-11149.Link

81. Chooi, Y.*, Wang, P., Fan, J., Li, Y., Wu, K., Wang, P., Tang, Y.“Characterization of a Group of Fungal Aromatic Polyketide Prenyl-transferases.” J. Am. Chem. Soc. 2012, 134, 9428–9437Link

80. Biswas, A., Liu, Y., Fan, G.*Tang, Y.* “Polyethylene Glycol-based Protein Nanocapsules for Functional Delivery of a Differentiation Transcription Factor” Biomaterials. 2012, 33, 5459–5467. Link

79. Wang, P., Gao, X., Tang, Y.* “Complexity Generation during Natural Product Biosynthesis using Redox Enzymes.” Curr. Opin. Chem. Biol. 2012, 16, 362-369. Link

78. Zhou, H., Gao, Z., Qiao, K., Wang, J., Vederas, J. C.*Tang, Y.* “A fungal ketoreductase domain that displays substrate-dependent stereospecificity.” Nat. Chem. Biol. 2012, 9, 331-333. (Cover Story) Link

77. Liu, Q., Yao, F., Chooi, Y., Kang, Q., Xu, W., Li, Y., Shao, Y., Shi, Y., Deng, Z., Tang, Y.*., You, D.* “Elucidation of Piericidin A1 Biosynthetic Locus Revealed an Thioesterase-dependent Mechanism of α-Pyridone Ring Formation.” Chem. Biol. 2012, 19, 243-253. Link

76. Winter, J. M., Tang, Y.“Synthetic Biological Approaches to Natural Product Biosynthesis.” Curr. Opin. Biotech. 2012, 5, 736-743Link

75. Zabala, A. O., Cacho, R., Tang, Y.* “Protein Engineering Towards Natural Product Synthesis and Diversification.” J. Ind. Microb. Biotechnol2012, 39, 227-241. (Invited Review) Link

74. Pickens, L. B., Sawaya, M. R., Pashkov, I., Rasool, H., Yeates, T. O., Tang, Y.* “Structural and Biochemical Characterizations of the GDSL Salicylyl-Acyltranferase SsfX3 from a Tetracycline Biosynthetic Pathway.” J. Biol. Chem. 2011, 286, 41539-41553 (selected as Paper of the Week). Link

73. Qiao, K. Chooi, Y., Tang, Y.* “Identification and Engineering of the Cytochalasin E Gene Cluster from Aspergillus clavatus.” Metab. Eng2011, 13, 723-732. Link

72. Li, Y., Chooi, Y., Sheng, Y., Valentine, J. S., Tang, Y.* “Comparative Characterization of Fungal Anthracenone and Naphthacenedione Biosynthetic Pathways Reveals an alpha-Hydroxylation-Dependent Claisen-Like Cyclization Catalyzed by a Dimanganese Thioesterase.” J. Am. Chem. Soc. 2011, 133, 15773-15785Link

71. Ames, B. D., Haynes, S. W., Gao, X., Evans, B. S., Kelleher, N. L., Tang, Y., Walsh, C. T.*, “Complexity Generation in Fungal Peptidyl Alkaloid Biosynthesis: Oxidation of Fumiquinazoline A to the Heptacyclic Hemiaminal Fumiquinazoline C by the Flavoenzyme Af12070 from Aspergillus fumigatus.” Biochemistry 2011, 50, 8756-8769.Link

70. Ames, B. D., Lee, M., Moody, C., Zhang, W., Tang, Y., Tsai, S. C.* “Structural and Biochemical Characterization of ZhuI Aromatase/Cyclase from the R1128 Polyketide Pathway.” Biochemistry 2011, 50, 8392–8406Link

69. Wang, P., Gao., X., Deng, Z., Tang, Y.* “Genetic Characterization of Enzymes Involved in the Priming Steps of Oxytetracycline Biosynthesis in Streptomyces rimosus.” Microbiology2011, 157, 2401-2409. Link

68. Haynes, S. W., Ames, B. D., Gao, X., Tang, Y., Walsh, C. T.* “Unraveling Terminal C-Domain-Mediated Condensation in Fungal Biosynthesis of Imidazoindolone Metabolites.” Biochemistry 2011, 50, 5668-5679. Link

67. Gu, Z., Biswas, A., Zhao, M., Tang, Y.* “Nanocarriers for Protein Intracellular Delivery.” Chem. Soc. Rev. 2011, 40, 3638-3655. Link

66. Zhao, M., Biswas, A., Hu, B., Joo, K., Wang, P., Gu, Z., Tang, Y.* “Redox-Responsive Nanocapsules for Intracellular Protein Delivery.” Biomaterials 2011, 32, 5223-5230. Link

65. Joo, K., Fang, Y., Liu, Y., Xiao, L., Gu, Z., Tai, A., Lee, C., Tang, Y., Wang, P.* “Enhanced Real-Time Monitoring of Adeno-Associated Virus Trafficking by Virus-Quantum Dot Conjugates.” ACS Nano 2011, 5, 3523-3535. Link

64. Gu, Z., Zhao, M., Sheng, Y., Bentolila, L. A. Tang, Y.* “Detection of Mercury Ion by Infrared-Fluorescent Protein and Its Hydrogel-Based Paper Assay.” Anal. Chem. 2011, 83, 2324-2329. Link

63. Qiao, K., Zhou, H., Xu, W., Zhang, W., Garg, N., Tang, Y.* “A Fungal Nonribosomal Peptide Synthetase Module that can Synthesize Thiopyrazines.” Org. Lett. 2011, 13, 1758-1761. Link

62. Gao, X., Chooi, Y., Ames, B. D., Wang, P., Walsh, C. T., Tang, Y.* “Fungal Quinazoline Alkaloid Biosynthesis: Genetic and Biochemical Investigation of the Tryptoquialanine Pathway in Penicillium aethiopicum.” J. Am. Chem. Soc. 2011, 133, 2729-2741. Link Highlighted by Nature Chem Biol.

61. Biswas, A., Joo, K., Zhao, M., Fan, G., Wang, P., Zhen, G., Tang, Y.* “Endoprotease-Mediated Intracellular Protein Delivery using Nanocapsules.”ACS Nano 2011, 5, 1385-1394. Link

60. Pickens, L., Tang, Y.*, Chooi, Y. H. “Metabolic Engineering for the Production of Natural Products” Annu. Rev. Chem. Bio. Eng. 20112, 211-236. (Invited Review) Link

59. Meehan, M. J.; Xie, X.; Zhao, X.; Xu, W.; Tang, Y.*; Dorrestein, P. C.* “FT-ICR-MS characterization of intermediates in the biosynthesis of the α-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF.” Biochemistry 2011, 50, 287–299. Link

58. Zhou, H., Qiao, K., Gao, Z., Vederas, J. C.*, Tang, Y.* “Insights into Radicicol Biosynthesis via Heterologous Synthesis of Advanced Intermediates and Analogs.” J. Biol. Chem2010, 285, 41412-4121. Link

57. Xu, W., Cai, X., Jung, M. E., Tang, Y.* “Analysis of Intact and Dissected Fungal Polyketide Synthase-Nonribosomal Peptide Synthetase in vitro and in Saccharomyces cerevisiae” J. Am. Chem. Soc. 2010, 132, 13604-13607. Link

56. Gao, X., Wang, P., Tang, Y.*, “Engineered polyketides biosynthesis and biocatalysis in Escherichia coli.” Appl. Microbiol. Biotechnol. 2010, 88, 1233-1242. (Invited Minireview) Link

55. Chooi, Y. H., Tang, Y.*, “Adding the Lipo to Lipopeptides: Do More with Less.” Chem. Biol. 2010, 17, 791-793. Link

54. Gu, Z., Biswas, A., Joo, K., Hu, B., Wang, P. and Tang, Y.*, “Probing Protease Activity with a Single Fluorescent Protein Nanobeacon.” Chem. Commun2010, 46, 6467-6469. Link

53. Pickens, L., Tang, Y.*, “Oxytetracycline Biosynthesis.” J. Biol. Chem2010, 285, 27509-27515 (Invited minireview). Link

52. Chooi, Y. H., Cacho, R., Tang, Y.*, “Identification of the Viridicatumtoxin and Griseofulvin Gene Clusters from Penicillium aethiopicum.” Chem. Biol. 2010, 17, 483-494. Link

51. Li, Y., Xu, W., Tang, Y.*, “Classification, Prediction and Verification of the Regioselectivity of Fungal Polyketide Synthase Product Template Domains.” J. Biol. Chem. 2010, 285, 22762-22771. Link Highlighted by Nature Chem Biol.

50. Gu, Z., Tang, Y.*, “Enzyme-Assisted Photolithography for Spatial Functionalization of Hydrogels.” Lab on Chip 2010, 10, 1946-1951. (FRONT COVER) Link

49. Zhou, H., Li, Y., Tang, Y.*, “Cyclization of Aromatic Polyketides from Bacteria and Fungi.” Nat. Prod. Rep2010, 27, 839-868. Link

48. Zhou, H., Qiao, K., Gao, Z.; Meehan, M. J., Li, J., Dorrestein, P. C., Vederas, J. C.*, Tang, Y.*, “Enzymatic Synthesis of Resorcylic Acid Lactones by Cooperation of Fungal Iterative Polyketide Synthases Involved in Hypothemycin Biosynthesis.” J. Am. Chem. Soc2010, 132, 4530–4531. Link

47. Yan, M, Du, J. J. Gu, Z., Liang, M., Hu, Y., Zhang, W., Segura, T., Liu, Z., Tang, Y. Lu, Y*. “A Novel Intracellular Protein Delivery Platform Based on Single-Protein Nanocapsules” Nat. Nano., 2010, 5.



46. Gu, Z., Yan, M., Hu, B., Joo, K., Biswas, A., Lu, Y.*, Wang, P.*, Tang, Y.* “Protein Nanocapsules Weaved by an Enzymatically Degradable Polymer Network.” Nano. Lett2009, 9, 4533-4538. Link

45. Gao, X., Xie, X., Pashkov, I., Sawaya, R. M., Laidmen, J., Zhang, W., Cacho, R., Yeates, T. O.*, Tang, Y.* “Directed Evolution and Structural Characterization of a Simvastatin Synthase.” Chem. Biol2009, 16, 1064-1074. Link

44. Pickens, L. B., Kim, W., Wang, P., Zhou, H., Watanabe, K., Gomi, S., Tang, Y.* “Biochemical Analysis of the Biosynthetic Pathway of an Anticancer Tetracycline SF2575.” J. Am. Chem. Soc2009, 131, 17677–17689. Link

43. Ma, S. A., Li, J., Choi, J. W., Zhou, H., Lee, M., Moorthie, V., Xie, X., Kealey, J. T., Da Silva, N. A., Vederas, J. C.*, Tang, Y.* “Complete Reconstitution of a Highly-Reducing Iterative Polyketide Synthase.” Science 2009, 326, 589-592. Link

42. Wang, M., Zhou, H., Wirz, M., Tang, Y., Boddy, C. N.* “A Versatile Thioesterase from an Iterative Fungal Polyketide Synthase.” Biochemistry 2009, 48, 6288-6290. Link

41. Xie, X., Meehan, M. J. Xu, W., Dorrestein, P. C., Tang, Y.* “Acyltransferase Mediated Polyketide Release From Fungal Megasynthase.” J. Am. Chem. Soc2009, 131, 8388-8389. Link

40. Wang, P., Zhang, W., Zhan, J., Tang, Y.* “Identification of OxyE as an Ancillary Oxygenase During Tetracycline Biosynthesis.” ChemBioChem2009, 10, 1554-1550. Link

39. Zhan, J., Qiao, K., Tang, Y.* “Investigation of Post-PKS Tailoring Modifications in Pradimicin Biosynthesis.” ChemBioChem2009, 10, 1447-1452. Link

38. Ma, S. M., Pickens, L. B., Tang, Y.* “Antibiotics, Polyketides.” Encyclopedia of Industrial Biotechnology2009, DOI: 10.1002/9780470054581.eib041.

37. Zhang, W., Tang, Y.“In vitro Analysis of Type II Polyketides.” Methods in Enzymology Volume: Complex Enzymes in Microbial Natural Product Biosynthesis. 2009, 459, 367-393Link

36. Pickens, L. B., Tang, Y. * “Decoding and Engineering Tetracycline Biosynthesis.” Metab. Eng2009, 1169-75. (Cover article)Link

35. Xie, X., Pashkov, I., Xue, G., Guerrero, J., Yeates, T., Tang, Y.* “Rational Improvement of Simvastatin Synthase Solubility in Escherichia coli Leads to Higher Whole-cell Biocatalytic Activity.” Biotechnol. Bioeng2009, 102, 20-28. Link

34. Zhang, W., Li, Y., Tang, Y. “Engineered Biosynthesis of Bacterial Aromatic Polyketides in Escherichia coli.” Proc. Natl. Acad. Sci. USA., 2008, 105, 20683-20685. Link

33. Zhou, H., Xie, X., Tang, Y.* “Engineering Natural Products Using Combinatorial Biosynthesis and Biocatalysis.” Curr. Opin. Biotechnol., 2008, 19, 590-596. Link

32. Zhan, J., Watanabe, K., Tang, Y.* “Synergistic Actions of Monooxygenase and Cyclases in Aromatic Polyketide Biosynthesis.” ChembioChem 2008, 9, 1710 – 1715. Link

31. Zhang, W., Watanabe, K., Cai, X., Jung, M. E., Tang, Y.*, Zhan, J.* “Identifying the Minimal Enzymes Required for Anhydrotetracycline Biosynthesis.” J. Am. Chem. Soc2008, 130, 6068-6069. Link

30. Zhou, H., Zhan, J., Watanabe, K., Xie, X., Tang, Y.* “A Polyketide Macrolactone Synthase from the Filamentous Fungus Gibberella zeae.” Proc. Natl. Acad. Sci. USA2008, 105, 6249-6254. Link

29. Ames, B., Korman, T., Zhang, W., Smith, P., Vi, T., Tang, Y., Tsai, S-C. “Crystal Structure and Functional Analysis of Tetracenomycin ARO/CYC: Implications for Cyclization Specificity of Aromatic Polyketides.” Proc. Natl. Acad. Sci. USA, 2008, 105, 5349-5354. Link

28. Ma, S. M., Zhan, J., Xie, X., Watanabe, K., Tang, Y.*, Zhang, W. “Redirecting the Cyclization Steps of Fungal Polyketide Synthase.” J. Am. Chem. Soc. 2008, 130, 38-39.Link

27. Zhang, W., Tang, Y.* “Combinatorial Biosynthesis of Natural Products” J. Med Chem. 2008, 51, 2629-2633. Link

26. Ma, S. M., Zhan, J., Watanabe, K., Xie, X., Zhang, W., Wang, C. C., Tang, Y.* “Enzymatic Synthesis of Aromatic Polyketides using PKS4 from Gibberella fujikuroi.” J. Am. Chem. Soc. 2007, 129, 10642-10643Link

25. Zhang, W.; Wilke, B. I.; Zhan, J.; Watanabe, K.; Boddy, C. N.*; Tang, Y.* “A New Mechanism for Benzopyrone Formation in Aromatic Polyketide Biosynthesis.” J. Am. Chem. Soc. 2007, 129, 9304-9305. Link.

24. Zhang, W., Watanabe. K., Wang, C. C. C., Tang, Y.* “Investigation of Early Tailoring Reactions in the Oxytetracycline Biosynthetic Pathway.” J. Biol. Chem. 2007, 282, 25717-25725. Link.

23. Xie, X., Wong, W. W., Tang, Y.* “Improving Simvastatin Bioconversion in Escherichia coli by Deletion of bioH.” Metab. Eng. 2007, 9, 379-386. Link

22. Ma, S. M., Tang, Y.* “Biochemical Characterization of the Minimal Polyketide Synthase Domains in the Lovastatin Nonaketide Synthase LovB.” FEBS J2007, 274, 2854–2864. Link

21. Xie, X., Tang, Y.* “Efficient Synthesis of Simvastatin Using Whole-Cell Biocatalysis.” Appl. Environ. Microb2007, 73, 2054-2060. Link

20. Zhang, W., Watanabe, K., Wang, C. C. C., Tang, Y.* “Heterologous Biosynthesis of Amidated Polyketides with Novel Cyclization Regioselectivity from Oxytetracycline Polyketide Synthase” J. Nat. Prod. 2006, 69, 1633-1636. Link

19. Xie, X., Watanabe, K., Wojcicki, W. A., Wang, C. C. C., Tang, Y.* “Biosynthesis of lovastatin analogs with a broadly specific acyltransferase.” Chem. Biol. 2006, 13, 1161-1169. Link

18. Zhang, W., Ames, B., Tsai, S-C., Tang, Y.*, "Tetracycline Biosynthesis: Sequencing of the Oxytetracycline Gene Cluster and Reconstitution of the Malonamyl-Specific Initiation module." Appl. Environ. Microb2006, 72, 2573-2580. link


Publication from Postdoctoral Work

17. Tang, Y. Lee, H.Y., Tang, Y., Kim, C-Y., Mathews, I. and Khosla, C. “Structural and Functional Studies on SCO1815: A -Ketoacyl-Acyl Carrier Protein Reductase from Streptomyces coelicolor A3(2).” Biochemistry2006, 45, 14085-14093.

16. Lee. T. S.*, Khosla, C., Tang, Y.* "Orthogonal Protein Interactions in Spore Pigment Producing and Antibiotic Producing Type II Polyketide Synthases." J. Antibiotics2005, 58, 664-667.

15. Lee. T. S.*, Khosla, C., Tang, Y.* "Engineered Biosynthesis of Aklanonic Acid Analogs." J. Am. Chem. Soc.2005, 127, 12254-12262.

14. Khosla, C., Tang, Y., "Chemistry. A new route to designer antibiotics." Science 2005, 308, 367-368.

13. Tang, Y., Koppisch, A., Khosla, C. "The Acyltransferase Homolog Found in Initiation Modules of Type II PKS is an Acetyl-ACP Thiolase that Suppresses Acetate-Priming of the KS-CLF." Biochemistry 2004, 43, 9546-9555.

12. Tang, Y., Lee, T. S., Lee, H. Y., Khosla, C. "Exploring the Biosynthetic Potential of Bimodular Aromatic Polyketide Synthases." Tetrahedron2004, 7659-7671.

11. Kumar, P.*, Khosla, C., Tang, Y.* "Manipulation and Analysis of Polyketide Synthases." Methods in Enzymology Volume 388: Protein Engineering 2004, 269-293.

10. Tang, Y., Lee, T. S., Khosla, C. "Engineered Biosynthesis of Regioselectively Modified Aromatic Polyketides Using Bimodular Polyketide Synthases." PLoS Biol. 2004, 2, 227-237.

9. Tang, Y., Tsai, S. C., Khosla, C. "Polyketide Chain Length Control by Chain Length Factor." J. Am. Chem. Soc. 2003, 125, 12708-12709.

8. Tang, Y., Lee, T. S., Kobayashi, S., Khosla, C. "Ketosynthases in the Initiation and Elongation Modules of Aromatic Polyketide Synthases Have Orthogonal Acyl-Carrier Protein Specificity." Biochemistry 2003, 42, 6588-6595.


Publication from Graduate Work

7. Tang, Y., Wang, P., Van Deventer, J. A., Link, A. J., Tirrell, D. A. “Introduction of an Aliphatic Ketone into Recombinant Proteins in a Bacterial Strain that Overexpresses an Editing-Impaired Leucyl-tRNA Synthetase.” ChembioChem 2009, 10, 2188-2190.

6. Cirino, P.C , Tang, Y., Takahashi, K., Tirrell, D. A., Arnold, F. H. "Global Incorporation of Norleucine in Place of Methionine in Cytochrome P450 BM-3 Heme Domain Increases Peroxygenase Activity." Biotechnol Bioeng. 2003, 20, 729-734.

5. Wang, P., Tang, Y., Tirrell, D. A., "Incorporation of Trifluoroisoleucine into Proteins In Vivo." J. Am. Chem. Soc. 2003, 125, 6900-6906.

4. Tang, Y., Tirrell, D. A. "Attenuation of Leucyl-tRNA Synthetase Proofreading Activity in Escherichia coli Allows Introduction of An Array of Unnatural Amino Acids in vivo." Biochemistry 2002, 41, 10635-10645.

3. Tang, Y., Tirrell, D. A. "Biosynthesis of Highly Stable Coiled-coils Containing Hexafluoroleucine Using an Engineered Bacterial Host." J. Am. Chem. Soc. 2001, 123, 11089-11090.

2. Tang, Y., Ghirlanda, G., Petka, W. A., Nakajima, T., W. A., DeGrado, W. F., Tirrell, D. A. "Fluorinated Coiled-coils Prepared in vivo Display Enhanced Thermal and Chemical Stability." Angew. Chem. Int. Ed. 2001, 40, 1494-1497.

1. Tang, Y., Ghirlanda, G., Vaidehi, N., Kua, J., Mainz, D. T., Goddard, W. A., DeGrado, W. F., Tirrell, D. A. "Stabilization of Leucine Zipper Coiled-coils using Trifluoroleucine." Biochemistry 2001, 40, 2790-2796.


Previous Book Chapters

6. Gu, Z.*, Tang, Y., Chen, Y., “Fabrication of Biomolecular Nanopatterns.” In Advances in Nanotechnology Volume III. eds Bartul, Z. and Jérôme Trenor, J.; NOVA scientific publisher, 2010.

5. Xie, X., Qiao, K. J., Tang, Y.* “Metabolic Engineering of Natural Product Biosynthesis.” In Bioactive Compounds from Natural Sources, Second Edition: Natural Products as Lead Compounds in Drug Discovery. ed Tringlai, C., Taylor and Francis Group, 2010.

4. Zhang, W., Ferreira, J. P., Tang, Y. "Regulation of Secondary Metabolism Biosynthesis." In: Smolke, C.D., ed. Handbook for Metabolic Pathway Engineering. CRC Press. 2009 in press.

3. Tang, Y.; Ma, S.M; Wojcicki, W. A. "Applications of Metabolic Engineering for Natural Drug Discovery." In: Smolke, C.D., ed. Handbook for Metabolic Pathway Engineering. CRC Press. 2009 in press.

2. Zhang, W., Tang, Y., "Engineering Starter Units in Aromatic Polyketides." In ACS Volume Based on Polyketides: Biosynthesis, Biological Activity and Genetic Engineering. eds S. R. Baerson; American Chemical Society, 2006.

1. Tang, Y., Khosla, C., "Diversity from Mother Nature: natural and unnatural products." In Exploiting Chemical Diversity for Drug Discovery, eds P. Bartlett and M. Entzeroth; Royal Society of Chemistry, 2006Link