Quantitative Video-Tracking Electrophoresis of Nanoemulsions

Seminar series
Physical Chemistry Seminar
Thu, Jun 13 12:00pm
2033 Young Hall
Speaker Xiaoming Zhu
University of California, Los Angeles
Dept. of Chemistry & Biochemistry

Abstract: Agarose gel electrophoresis is a standard method used to separate and identify nucleic acids as well as other nanoscale structures, such as viruses. We use video band-tracking to analyze agarose gel electrophoresis of charged polystyrene nanosphere standards, and we measure the dependence of the band propagation velocity on the gel concentration, the applied electric field strength, and the pH of the buffer. After optimizing the gel electrophoresis conditions, we study the dependence of the band velocity and the scattered light intensity on the average droplet radius and on the particle volume fraction. Our study gives rise to a quantitative model describing how spheres move through the elastic, porous agarose gel medium when driven by an electric field. Using these relationships, we optimize colloidal separation and fractionation using agarose gel electrophoresis.