Directed Evolution for Commercial Enzymes

Seminar series
Organic Colloquium
Thu, May 30 2:00pm
Cram Conference Room – 3440 Mol Sci
Speaker Dr. Gjalt W. Huisman
Vice President, Pharmaceutical R&D
Codexis, Inc.

Special Chemistry C105/CM205A–Organic Colloquium–Chemistry-Biology Interface Lecture

“Directed Evolution for Commercial Enzymes”

Abstract.  Using advanced enzyme engineering technology Codexis has developed practical, green manufacturing processes for a range of pharmaceutical intermediates and APIs.  Our 4th generation enzyme engineering technology has produced a diverse array of enzymes that are operationally equivalent and complementary to the “standard” catalysts in the chemists’ tool box.  Parallel optimization of enzyme, enzyme formulation, and chemical process continues to push the catalyst cost contribution lower, expanding the application of custom-made enzymes into larger volume opportunities.

Following an introduction on the technology, examples of the deployment of such biocatalyst in the commercial manufacture of intermediates and active pharmaceutical ingredients will be presented, including instances where there are no chemical equivalents to the enzymatic reaction.  Chemistries that will be highlighted include the asymmetric reduction of ketones, oxidation of amines and sulfides for the manufacture of chiral amines and sulfoxides, as well as transamination of ketones to chiral amines.