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DTSTART;TZID=America/Los_Angeles:20240122T160000
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SUMMARY:Physical Chemistry Seminar 228: Giulia Palermo
DESCRIPTION:Prof. Giulia Palermo Flyer \nTitle: Dynamics and mechanisms of CRISPR-Cas9 through the lens of computational methods \nAbstract: The clustered regularly interspaced short palindromic repeat (CRISPR) genome-editing revolution established the beginning of a new era in life sciences. I will report the role of state-of-the-art computations in the CRISPR-Cas9 revolution\, from the early refinement of cryo-EM data to enhanced simulations of large-scale conformational transitions. Molecular simulations reported a mechanism for RNA binding and the formation of a catalytically competent Cas9 enzyme\, in agreement with subsequent structural studies. Inspired by single-molecule experiments\, molecular dynamics offered a rationale for the onset of off-target effects\, while graph theory unveiled the allosteric regulation. Finally\, the use of a mixed quantum-classical approach established the catalytic mechanism of DNA cleavage. Overall\, molecular simulations are instrumental in understanding the dynamics and mechanism of CRISPR-Cas9 and pave the way for a new field of computational studies aimed at deciphering and engineering genome editing systems.
URL:https://www.chemistry.ucla.edu/events/physical-chemistry-seminar-228-giulia-palermo/
LOCATION:Collaboratory Yoo Seminar & Conference Hall YH4222 
CATEGORIES:Physical,Physical Chemistry Seminar
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